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The Hampden Model H-6517 Aeration Unit has been developed to investigate oxygen transfer characteristics of diffused air systems along with physical and chemical parameters which effect oxygenation in terms of retention time. The level of Dissolved Oxygen (DO) in water depends on many physical, chemical and biochemical activities in the sample. The most important factors to consider are pressure, temperature and salinity. The amount of oxygen a sample can hold in a solution will vary with the temperature of the sample, the pressure or altitude at which the sample is measured and the concentration of salts dissolved in the sample. The analysis of DO is one of the key tests performed in water pollution and waste water treatment studies.